Doxycycline Hyclate is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections.
Doxycycline hyclate (10 mg) was dissolved in 40 gdispersion solution of CS-PNIPAAm NPs at pH 4.5 and 25 ℃. After incubation for 96 h, the dispersion solution was adjusted to pH 9.0. Drug-loaded NPs were separated from the solution by centrifugation at 13,500 rpm and 20 ℃ for 5 min. The drug-loaded CS-PNIPAAm NPs were dried by lyophilization and then weighed. Doxycycline hyclate concentration in the supernatant was analyzed by measuring the UV absorbance at the wavelength of 346 nm. The amount of the drug loaded in the NPs thus could be calculated by subtracting the amount of drug in the supernatant from the feed.
19 Sep 2016, 3:08 am
