Can anyone help me with this one (WP having a lot of clever people, and yes, I've tried google already)
I’ve been told that with the data from my experiment I should be able to arrive at
i) enzyme activity in soln at zero time in nanokatals/gramme of tissue
And
ii) Enzyme activity in soln at 1 hour nanokatals/gramme of tissue.
Then I should from these figures be able to work out percentage enzyme leakage.
My experiment, well it was :-
adding a substance over standard ranges to liver slices,
incubate 1 hour to biotransform,
then add NADH, alanine and alpha ketoglutarate,
spectroscopy for 3mins to watch production of pryruvate oxidise NADH to NAD(because of differing absorbancy levels). (also zero time control done, predictably flat line)
All fine and good, the biochemistry makes sense, reactions worked..got data
I’m just a dunce with equations. And overthinking it now, which got me more confused
I Can do Beers law to get rate on the slope...fair enough. (although I don’t see how that helps with anything )
But how do I get my nano katals values? I’m lost. So any help very gratefully appreciated. Otherwise I might actually have to TALK to academic staff .....
Honestly, why can’t they let me stick with immunostaining and western blots?
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Other people are people too.
24 Apr 2009, 4:12 pm
